Eventos antioxidantes asociados a la elicitación de suspensiones celulares de Thevetia peruviana para la producción de compuestos cardiotónicos

  • Carlos Julio Nova López Facultad de Ciencias, Universidad Nacional de Colombia
Palabras clave: T. peruviana, Glucósidos cardiotónicos, Peruvósido, EROS

Resumen

Thevetia peruviana es una planta con capacidad de síntesis de glicósidos con actividad cardiotónica. El cultivo en suspensión de estas células vegetales es una alternativa para la producción, bajo condiciones controladas y reproducibles a escala industrial, de estos compuestos. En estos cultivos se ha reportado previamente la obtención de peruvósido, un glicósido cardiotónico que resulta de especial interés porque genera menos reacciones alérgicas en los pacientes, en comparación con otras moléculas de la misma naturaleza. En este trabajo, se estudiaron los eventos antioxidantes asociados a la elicitación de cultivos de células en suspensión con metil jasmonato (meJa) (100 mg l-1). Se evaluó la producción de peruvósido, la viabilidad celular, la producción de H2O2, la actividad de la enzima guayacol peroxidasa (G-POD) y la producción de especies reactivas de oxígeno totales (EROS). La adición de MeJa promovió la síntesis de peruvósido y estimuló la reducción en los niveles de H2O2 mediante la promoción de la actividad de la guayacol peroxidasa. Los resultados de este estudio contribuyen a comprender los eventos bioquímicos en el cultivo de células en suspensión bajo condiciones de elicitación, constituyéndose en un aporte para la producción in vitro de compuestos bioactivos.

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Publicado
2018-12-28
Cómo citar
Nova López, C. J. (2018). Eventos antioxidantes asociados a la elicitación de suspensiones celulares de Thevetia peruviana para la producción de compuestos cardiotónicos. Ciencia, Tecnología E Innovación En Salud, 3, 30 - 43. https://doi.org/10.23850/25393871.2127