Obtaining bacterial cellulases using vegetable waste from marketplaces

Abstract

Market places generate organic waste that can be valued by including it in biotechnological production chains such as the production of enzymes from low-cost substrates. The objective of this study was to obtain bacterial cellulases from vegetable residues generated in the El Restrepo marketplace - Bogotá. A substrate was formulated using vegetable residues and inoculated with the C6M2 bacterial strain, monitoring the enzymatic activity, the content of reducing sugars and the residual cellulose for 72 hours. The highest enzymatic activity was observed at 33 hours (22.40 U/mL). During fermentation, a decrease in reducing sugars was observed from 0.43 g/mL to 0.032 g/mL and residual cellulose from 92.5% to 3.1%. This allows us to conclude that vegetable waste can be included in circular economy processes as substrates for obtaining cellulases.