Extraction and identification of pepper (Capsicum annuum) farming associate microorganisms at La Mesa (Cundinamarca

Abstract

It was an experiment conducted in laboratory and field conditions, with the aim to extract and identify beneficial microorganisms associated with the cultivation of pepper (Capsicum annuum) in the town of La Mesa (Cundinamarca). This project consisted of two phases: extraction and identification. During the extraction process of microorganisms, three previously sterilized rice traps were installed in three pepper plants healthy and productive state (for a total of 9 traps). Additionally, two traps rice (absolute control) stored in the shade and were left unused; periodically checking that no microorganisms grow on them to show that the sterilization process was correct. The setting of traps was randomized for each plant. It was demonstrated that microorganisms extraction methodology works properly and which is additionally easily accessible for farmers. Strains of Trichoderma sp. fungi were found (beneficial microorganisms) and Fusarium sp. (optional pathogen). The strain of Trichoderma sp. obtained from the municipality of La Mesa was identified by the Institute of Genetics at the National University of Colombia through an examination of DNA (deoxyribonucleic acid). This research will allow farmers in the municipality of La Mesa playing a beneficial fungus for the agricultural sector, which is adapted to the soil conditions (acidity, texture, humidity, among others) and climate (temperature, precipitation, altitude, etc.) of this country.