Isolation of Trichoderma sp., In agricultural production units Agribusiness Training Center La Angostura Campoalegre (Huila)

Abstract

Abstract: Antagonistic fungi have become major alternatives to the biological control of plant pathogens in the soil. Trichoderma is the genus of fungi that is considered one of the most widely used as an agent of fungal control; establishing symbiosis with plants, in some cases acting as antagonists of plant pathogenic fungi and bacteria as Fusarium sp, Curvularia sp, Pseudomonas, among others, invertebrates and even weeds. Hyperparasitism, antibiosis and / or nutrient competition are typical biocontrol actions of these fungi. In this sense, the objective of this study was to isolate the fungus Trichoderma from the agricultural productive units of the Agro-industrial Training Center La Angostura, located in the town of Campoalegre in the department of Huila.
The study was conducted between April and September, 2014. This work was developed in two phases: the first phase was carried out on the field, where soil sampling methodology and trapping using rice as substrate was used. In the second phase, which included lab work, samples were taken to make serial dilutions on base 10 for sowing set as well as seed granules were sowed in the PDA crop, Sabouraud and Rose Bengal. Through this latter method, that is using Rose Bengal Agar, it was possible to obtain the growth of velvety greenish-white colonies, the distinctive color of the fungus. This is a starting point to perform future in vitro antagonistic tests with fungal pathogens that cause diseases such as Fusarium; in order to produce an effective bio-input adapted to the agro-ecological conditions of the area.