Abstract
In the present research, an analytical methodology to micro scale based on the use of the HS-SPME/GC-MS to determine volatile compounds present in Clinopodium gilliesii (Benth.) Kuntze (Lamiaceae) was employed, and settled differences and similarities with its essential oil obtained by hydrodistillation. A systematic description of the volatile components of flowers, stems, leaves and combined aerial parts (whole plant) was constructed via GC-MS analyses of HS-SPME adsorbed compounds and of essential oils obtained through hydrodistillation of the same tissues. Piperitenone oxide and piperitone oxide were the main components of both the HS-SPME analysis and essential oil analysis. The HS-SPME method can achieve comparable results to those obtained by essential oil analysis, by using very fewer samples, a shorter extraction time and a much simpler procedure.
Para citar este artículo
Vázquez, A., Aimar, M.,Decarlini, M., Demmel, G., Cantero, J y Ruiz, G. (2016). Volatile Organic Constituents of Clinopodium gilliesii (Benth.) Kuntze: Analysis by HS-SPME and classic hydrodistillation. Rev. Colomb. Investig. Agroindustriales, 3(1), 91-100. DOI: http://dx.doi.org/10.23850/24220582.351
References
Angioni, A., Barra, A., Coroneo, V., Dessi, S. & Cabras, P. (2006). Chemical composition, seasonal variability and antifungal activity of Lavandula stoechas L. ssp. stoechas essential oils from stem/leaves and flowers. J Agric Food Chem, 54 (12), 4364–4370. http://dx.doi.org/10.1021/jf0603329
Babu, K.G.D. & Kaul, V.K. (2007). Variations in quantitative and qualitative characteristics of wild marigold (Tagetes minuta L.) oils distilled under vacuum and at NTP. Ind Crops Prod, 26(3), 241 - 250. http://dx.doi.org/10.1016/j.indcrop.2007.03.013
Barboza, G.E., Cantero, J.J., Núñez, C.O. & Ariza-Espinar, L. (2006). Flora Medicinal de la Provincia de Córdoba (Argentina): Pteridófitas y Antofitas silvestres o naturalizadas. “Lamiaceae”. Argentina: First Edition. Museo Botánico Córdoba.
Goel, D., Singh, V., Ali, M., Mallavarupu, G.R. & Kumar, S. (2007). Essential oils of petal, leaf and stem of the antimalarial plant Artemisia annua. J Nat Med, 61,187. http://dx.doi.org/10.1007/s11418-006-0112-9
Hnatyszyn, O., Moscatelli, V., García, J., Rondina, R., Costa, M., Arranz, C., Balaszczuk, A., Ferraro, G. & Coussio, J.D. (2003). Argentinian plant extracts with relaxant effect on the smooth muscle of the corpus cavernosum of guinea pig. Phytomedicine, 10(8), 669 – 674. http://dx.doi.org/10.1078/0944-7113-00261
Kothari, S.K., Bhattacharya, A.K., Ramesh, S., Garg, S.N. & Khanuja, S.P.S. (2005). Volatile Constituents in Oil from Different Plant Parts of Methyl Eugenol-Rich Ocimum tenuiflorum L.f. (syn. O. sanctum L.) Grown in South India. J Essent Oil Res, 17 (6), 656-658. http://dx.doi.org/10.1080/10412905.2005.9699025
Kovacevic, N.N., Marcetic, M.D., Lakusic, D.V. & Lakusic, B.S. (2016). Composition of the Essential Oils of Different Parts of Seseli annuum L. (Apiaceae), J Essent Oil Res, 19(3), 671-677. http://dx.doi.org/10.1080/0972060X.2014.901604
Mohammadhosseini, M., Akbarzadeh, A. & Hashemi-Moghaddam, H. (2016). Gas Chromatographic-Mass Spectrometric Analysis of Volatiles Obtained by HSSPME-GC-MS Technique from Stachys lavandulifolia and Evaluation for Biological Activity: A Review. J Essent Oil Bear Pl, 19(3), 671-677. http://dx.doi.org/10.1080/0972060X.2016.1221741
Mohammadhosseini, M., Mahdavi, B., & Akhlaghi, H. (2013). Characterization and chemical composition of the volatile oils from aerial parts of Eryngium bungei Bioss. (Apiaceae) by using traditional hydrodistillation, microwave assisted hydrodistillation and head space solid phase microextraction methods prior to GC and GC/MS analyses: a comparative approach. J Essent Oil Bear Pl, 16(5), 613-623. http://dx.doi.org/10.1080/0972060X.2013.854484
Mohammadhosseini, M. (2015a). Chemical composition of the volatile fractions from flowers, leaves and stems of salvia mirzayanii by HS-SPME-GC-MS, J Essent Oil Bear Pl, 18(2), 464-476. http://dx.doi.org/10.1080/0972060X.2014.1001185
Mohammadhosseini, M. (2015b). Chemical composition of the essential oils and volatile fractions from flowers, stems and roots of salvia multicaulis Vahl. By using MAHD, SFME and HS-SPME methods. J Essent Oil Bear Pl, 18(6), 1360-1371. http://dx.doi.org/10.1080/0972060X.2015.1024447
Oliveira, T.L.C., Soares, R.A., Ramos, E.M., Cardoso, M.G., Alves, E. & Piccoli, R.H. (2011). Antimicrobial activity of Satureja montana L. essential oil against Clostridium perfringens type A inoculated in mortadellatype sausages formulated with different levels of sodium nitrite. Int J Food Microbiol, 144(3), 546-555. http://dx.doi.org/10.1016/j.ijfoodmicro.2010.11.022
Radulović, N., Dekić, M., Stojanović-Radić, Z. & Palić, R. (2009). Volatile constituents of Erodium cicutarium (L.) L’ Hérit. (Geraniaceae). Cent Eur J Biol, 4(3), 404–410. http://dx.doi.org/ 10.2478/s11535-009-0026-0100
Rajeswara, R. B.R., Adinarayana, G., Rajput, D.K., Kumar, A.N. & Syamasundar, K.V. (2015). Essential oil profiles of different parts of East Indian lemongrass (Cymbopogon flexuosus [Nees ex Steud]. Wats.), J Essent Oil Res, 27(3), 225-231.
Santos, F.M., Pinto, J.E.B.P., Bertolucci, S.K.V., Alvarenga, A.A., Alves, M.N., Duarte, M.C.T. & Sartoratto, A. (2013). Chemical composition and antimicrobial activity of the essential oil from the leaves and flowers of Aloysia gratissima. Rev Bras Plantas Med, 15(4), 583-588. https://dx.doi.org/10.1590/S1516-05722013000400015
Saroglou, V., Dorizas, N., Kypriotakis, Z. & Skaltsa, H.D. (2006). Analysis of the essential oil composition of eight Anthemis species from Greece. J Chromatogr A, 1104(1-2), 313- 322. http://dx.doi.org/10.1016/j.chroma.2005.11.087
Shahid, Ud-Daula, A.F.M., Demirci, F., Salim, K.A., Demirci, B., Lim, L.B.L., Baser, K.H.C. & Ahmad, N. (2016). Chemical composition, antioxidant and antimicrobial activities of essential oils from leaves, aerial stems, basal stems, and rhizomes of Etlingera fimbriobracteata (K.Schum.) R.M.Sm. Industrial Crops and Products, 84, 189–198. http://dx.doi.org/10.1016/j.indcrop.2015.12.034
Vázquez, A.M., Aimar, M.L., Demmel, G.I., Cabalen, M.E., Decarlini, M.F., Cantero, J.J, Criado, S.G. & Ruiz, G.M. (2014). Identification of volatile compounds of Clinopodium odorum (Lamiaceae): A comparison between HS-SPME and classic hydrodistillation. Bol Latinoamer Caribe Plant Med Arom 13(3), 285-296.
Viturro, C.I., Molina, A.C., Heit, C., Elechosa, M.A.,Molina, A.M. & Juárez, M.A. (2007). Evaluación de la composición de los aceites esenciales de Satureja boliviana, S. odora y S. parvifolia, obtenidos de colectas en Tucumán, Argentina. Bol Latinoam Caribe Plant Med, Aromaticas, 6(5), 288-289.
Wesolowska, A. & Jadczak, A. (2016). Composition of the essential oils from inflorescences, leaves and stems of Ocimum basilicum “Cinnamon” cultivated in northwestern Poland. J Essent Oil Bear Pl, 19(4), 1037-1042. http://dx.doi.org/10.1080/0972060X.2016.1197801