Keywords
hydroxyapatite
bone grafts
xenograft
extracellular matrix
osteoblasts hidroxiapatita
injertos óseos
xenoinjerto
matriz extracelular
osteoblastos
bone grafts
xenograft
extracellular matrix
osteoblasts hidroxiapatita
injertos óseos
xenoinjerto
matriz extracelular
osteoblastos
How to Cite
Payán Valero, A., Moreno Cepeda, Y., Gil Bedoya, J. P., Grueso Ruiz, L., Guzmán Valencia, J., Lozano Nieva, K. J., Pustovrh Ramos, M. C., & Valencia Llano, C. H. (2018). Cellular culture of a bovine apatite as a bone substitute: preliminar tests. Informador Tecnico, 82(2), 172–180. https://doi.org/10.23850/22565035.1376
Abstract
The bone loss limits the possibility of dental rehabilitation, being necessary in many occasions to carry out bone reconstruction process for the placement of intraosseous implants and to improve the prosthetics profiles. The autologous
bone is the ideal substitute, but there are other alternatives such as tissue of donor origin (homologous), animal origin (xenologous) and synthetic origin (alloplastic). At the engineering school of materials of the university of Valle, a bone
substitute is being developed from bovine hydroxyapatite, which was obtained from veal bones previously washed (to eliminate fat and soft tissues) provided by the beef industry. These bones were fractionated, ground, and submitted to a
thermal treatment up to 800 celsius degrees.The samples were characterised by X ray diffraction and fourier transformed infrared spectroscopy (FTIR). In this paper, the osteoconduction of the material was evaluated; for this purpose, 15 samples
were submitted to preliminary tests and then cultured with osteoblasts for 15 days.The surface characteristics of these samples were determined by scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS), these
characteristics determined cellular functions such as adhesion, maturation and extracellular matrix formation.The results revealed adhesion and cellular growth, as well as the presence of deposits that are compatible with extracellular osseous
matrix and that disclose organic and inorganic contents, which is an indicator for maturing.
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