Abstract
In the present study, the biological quality and cell viability of different microbial inoculants marketed in Baja California, Mexico, was carried out. For this purpose, the antagonistic capacity was determined in triplicate by means of dual confrontation with phytopathogenic fungi, determination of cell viability using the serial dilution technique in culture medium; as well as the review of compliance with the packaging criteria based on the Mexican regulations in the products: Bioben, T-22, Funqui, Agroderma, Bio-Tilis, Enerbac, Fus-Out and Bacillus 1537. The results showed that the products Agroderma, Bacillus 1537 and Fus-Out, no health registration submitted. On the other hand, the viability of the inoculants evaluated by means of the plate count technique, showed values of CFU/g or CFU/mL, lower than those indicated by the labels of the commercial inoculants. The results of antagonism of biological fungi against phytopathogenic fungi: Alternaria alternata, Macrophomina sp., Fusarium solani, and Botrytis sp., showed that only the product formulated based on Bacillus 1537 presented an inhibitory effect greater than 50% on phytopathogens. evaluated. In contrast, the products formulated based on mycoparasitic fungi, showed a lower efficiency of inhibition of phytopathogenic fungi, being the Funqui inoculant, the one that presented the capacity to inhibit their growth by 50%. Finally, Baja California has a wide presence of commercial inoculants; however, its quality and biological efficiency is variable against the pathogens present in the agricultural soils of the region.
References
Aloo, B. N., Mbega, E. R., Makumba, B. A. y Tumuhairwe, J. B. (2022). Effects of carrier materials and storage temperatures on the viability and stability of three biofertilizer inoculants obtained from potato (Solanum tuberosum L.) rhizosphere. Agriculture, 12 (2): 140. https://doi.org/10.3390/agriculture12020140
Amatuzzi, R.F., Cardoso, N., Poltronieri, A.S., Poitevin, C.G., Dalzoto, P., Zawadeneak, M.A. y Pimentel, I.C. (2018). Potential of endophytic fungi as biocontrol agents of Duponchelia fovealis (Zeller) (Lepidoptera:Crambidae). Brazilian Journal of Biology, 78 (3):429-435. https://dx.doi.org/10.1590/1519-6984.166681
Avendaño, B. y Varela, R. (2010). La adopción de estándares en el sector hortícola de Baja California, México. Estudios Fronterizos, Nueva Época. 21(11):171-202. https://doi.org/10.21670/ref.2010.21.a06
Benintende, S. (2010). Calidad de inoculantes comerciales para el cultivo de soja en la Argentina: concentración de rizobios viables y presencia de contaminantes. Revista Argentina de Microbiología, 42 (2): 129-132. https://www.redalyc.org/articulo.oa?id=213014892013
Cornell C., Kokkoris V., Richards, A., Horst, C., Rosa, D., Bennett, J.A. y Hart, M.M. (2021). Do Bioinoculants Affect Resident Microbial Communities? A Meta-Analysis. Frontier Agronomy, 3: 753474. https://doi.org/10.3389/fagro.2021.753474
Gómez M.A., Silva, N., Hartmann A., Sagardoy, M.A. y Catroux, G. (1997). Evaluation of commercial soybean inoculants from Argentina. World Journal of Microbiology and Biotechnology. 13:167–173. https://doi.org/10.1023/A:1018533629378
González-Mendoza, D., Torrentera-Olivera, N.G., Ceceña-Duran, C. y Grimaldo-Juarez O. (2015). Water as contamination source of Salmonella and Escherichia coli in vegetable production in Mexico: A review. Revista Bio Ciencias, 3:156-162. http://dx.doi.org/10.15741/revbio.03.03.02
González-Soto, T.E., Moreno-Ramírez, L., Troncoso-Rojas, R., González-Mendoza, D., Sánchez-Estrada, A., Grimaldo Juárez, O., Tzinzun-Camacho, O. y Ceceña-Durán, C. (2017). Inoculación de Trichoderma longibrachiatum en algodón transgénico: Cambios en compuestos fenólicos y enzimas de estrés oxidativo. Idesia (Arica) 35 (1): 19-24. http://dx.doi.org/10.4067/S0718-34292017005000005
Han, H.S., Supanjani E. y Lee, K.D. (2006). Effect of co-inoculation with phosphate and potassium solubilizing bacteria on mineral uptake and growth of pepper and cucumber. Plant Soil Environment, 52 (3):130–136. https://doi.org/10.17221/3356-PSE
Kunova, A., Bonaldi, M., Saracchi, M., Pizatti, C., Chen, X. y Cortesi, P. (2016). Selection of Streptomyces against soil borne fungal pathogens by a standardized dual culture assay and evaluation of their effects on seed germination and plant growth. BMC Microbiology, 16: 272. https://doi.org/10.1186/s12866-016-0886-1
Maciel, C. G., Walker, C., Muniz, M.F. y Araújo, M. M. (2014). Antagonismo de Trichoderma SPP. E Bacillus subtilis (UFV3918) a Fusarium sambucinum em Pinus elliottii engelm. Revista Árvore, 38 (3): 505-512. https://doi.org/10.1590/S0100-67622014000300013
Matar, S.M., El-Kazzaz, S.A., Wagih E.E., El-Diwany, A.I., Moustafa, H.E., Abo-Zaid, G.A., Abd-Elsalam, H.E. y Hafez, E.E. (2009). Antagonistic and inhibitory effect of bacillus subtilis against certain plant pathogenic fungi. Biotechnology, 8 (1): 53-61. https://doi.org/10.3923/biotech.2009.53.61
Moreno-Ramírez, L., González-Mendoza, D., Cecena-Duran, C. y Grimaldo-Juarez, O. (2015). Molecular identification of phosphate-solubilizing native bacteria isolated from the rhizosphere of Prosopis glandulosa in Mexicali valley. Genetic and Molecular Research, 14 (1):2793-2798. https://doi.org/10.4238/2015.March.31.9
Timmusk, S., Behers, L., Muthoni, J., Muraya, A. y Aronsson, A.C. (2017). Perspectives and challenges of microbial application for crop improvement. Frontiers in Plant Science, 8 (49). http://doi.org/10.3389/fpls.2017.00049
Tranier, M.S., Pognant-Gros, J., De la Quiroz, R., Aguilar, C.N., Mateille, T. y Roussos, S. (2014). Commercial biological control agents targeted against plant-parasitic root-knot nematodes. Brazilian Archives of Biology and Technology. 57(6) :831-841. https://doi.org/10.1590/S1516-8913201402540
Vargas-Bejarano, E., Méndez-Trujillo, V., Vázquez-Angulo, J.C., González-Mendoza, D. y Grimaldo-Juarez, O. (2012). Cambios fisiológicos en algodón transgénico inoculado con Trichoderma spp. Phyton (Buenos Aires), 81(1):101-105. http://www.revistaphyton.fund-romuloraggio.org.ar/vol81/13-VARGAS-BEJARANO.pdf
Younesi, H., Bazgir, E., Darvishnia, M. y Chehri, K. (2021). Selection and control efficiency of Trichoderma isolates against Fusarium oxysporum f. sp. ciceris in Iran. Physiological and Molecular Plant Pathology, 116 :101731. https://doi.org/10.1016/j.pmpp.2021.101731
Zambrano-Moreno, D.C., Ramón-Rodríguez, L.F., Van Strahlen-Pérez, M. y Bonilla-Buitrago, R.R. (2015). Industria de bioinsumos de uso agrícola en Colombia. Revista U.D.C.A Actualidad & Divulgación Científica, 18 (1): 59-67. https://doi.org/10.31910/rudca.v18.n1.2015.445
Zlolniski, C. (2011). Water flowing north of the border: export agriculture and water politics in a rural community in Baja California. Cultural Anthropology, 26:565-88. https://doi.org/10.1111/j.1548-1360.2011.01112.x

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